Berg showed early artistic talents, attending Pratt Institute when he was 12 years old, and later studying at Cooper Union. He served a period of time in the Army Air Corps. In 1940, he joined Will Eisner's studio, where he wrote and drew for the Quality Comics line. Berg's work also appeared in Dell Comics and Fawcett Publications, typically on humorous back-up features. Beginning in the mid-1940s, he worked for several years with Stan Lee on comic books at Timely Comics (now known as Marvel Comics), ranging from Combat Kelly and The Ringo Kid to Tessie the Typist. He also freelanced for a half-dozen other comic companies.
Berg's other work included the comic strips Citizen Senior (1989-93), Roger Kaputnik (1992) and Astronuts (1994). He also produced regular religious-themed work for "Moshiach Times" and the B'nai Brith newsletter and held an honorary doctorate in theology. His interaction with Mad's atheist publisher Bill Gaines was suitably irreverent: Berg would always tell Gaines "God bless you," and Gaines would reply, "Go to Hell."
His characters occasionally made their way into other artists' works, such as Kaputnik finding himself a patient in a Mort Drucker spoof of Saint Elsewhere, along with the words "with apologies to Dave Berg".
Berg contributed to Mad until his death, a total of 46 years. His last set of "Lighter Side" strips, which had been written but not penciled, were illustrated after Berg's death by 18 of Mad's other artists as a final tribute.
Recently, Dave Berg's Lighter Side strips have begun to be re-written with updated jokes for Mad by long time Mad writer, Dick DeBartolo, among others.
He died in his home in Marina del Rey, California, shortly after midnight on May 17, 2002 after a long battle with cancer, with his wife of 52 years, Vivian, and their two children by his side.