DNA-(apurinic or apyrimidinic site) lyase

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate

This enzyme belongs to the family of lyases, specifically the "catch-all" class of lyases that cleave carbon-oxygen bonds. The systematic name of this enzyme class is DNA-(apurinic or apyrimidinic site) 5'-phosphomonoester-lyase. Other names in common use include AP lyase, AP endonuclease class I, endodeoxyribonuclease (apurinic or apyrimidinic), deoxyribonuclease (apurinic or apyrimidinic), E. coli endonuclease III, phage-T4 UV endonuclease, Micrococcus luteus UV endonuclease, AP site-DNA 5'-phosphomonoester-lyase, and X-ray endonuclease III.

Structural studies

As of late 2007, 23 structures have been solved for this class of enzymes, with PDB accession codes , , , , , , , , , , , , , , , , , , , , , , and .

References

• Bailly V, Sente B, Verly WG "Bacteriophage-T4 and Micrococcus luteus UV endonucleases are not endonucleases but beta-elimination and sometimes beta delta-elimination catalysts". Biochem. J. 259 751–9.
• Bailly V, Verly WG "Escherichia coli endonuclease III is not an endonuclease but a beta-elimination catalyst". Biochem. J. 242 565–72.
• Bailly V, Verly WG "AP endonucleases and AP lyases". Nucleic. Acids. Res. 17 3617–8.
• PH "Mechanism of UV endonuclease-V cleavage of abasic sites in DNA determined by C-13 labeling". J. Am. Chem. Soc. 110 2690–2691.

External links

The CAS registry number for this enzyme class is .

Gene Ontology (GO) codes