Near 445 BC, her husband Megabyzus started a successful revolt in Syria against Artaxerxes I. Initially, Amytis stayed with the king during the war; however, she later participated, along with Amestris and the satrap Artarius, in the reconciliation negotiations between the rebel and the king. Notwithstanding this, Megabyzus again fell in disgrace and was expelled from the court and exiled to a town on the Persian Gulf. After five years in exile, Magabyzus was forgiven and allowed to return to the court, again thanks to the intercession of Amytis and Amestris.
Amytis bore Megabyzus two sons: Zopyrus and Artyphius. After the death of his father and mother, Zopyrus fled to Athens, where, according to Ctesias, he "was well received owing to the services his mother had rendered to the Athenians".
Greek sources portray Amytis as a licentious woman. According to Ctesias, during Xerxes' reign she was accused of adultery by Megabyzus. The same historian further affirms that, after her husband's death, she had a love affair with the Greek physician Apollonides of Cos, and that when the affair was discovered, Apollonides was tortured and put to death by queen mother Amestris. Dinon, another Greek historian, describes Amytis as the most beautiful and licentious woman of Asia. The most difficult challenge in using historians as Ctesias or Dinon as reliable sources is the fact that they tended to write amazing stories that would better appeal to their readers, often without much attention to historical rigor. The lack of primary sources makes it therefore impossible to have an accurate image of Amitis.