Acuitzío is a municipality located in the central region of the Mexican state of Michoacán. The municipality has an area of 180.13 square kilometres (0.30% of the surface of the state) and is bordered to the north and northwest by the municipality of Morelia, to the east by Madero, to south by Tacámbaro, and to the southwest by Huiramba. The municipality had a population of 10,052 inhabitants according to the 2005 census Its municipal seat is the city of Acuitzío del Canje.

A religious center near Acuitzío named Coatepec was founded by the Aztecs sometime in the 12th century. It is believed that the Aztecs abandoned the center to migrate to a place their Gods had mandated. Acuitzío is a word of Tarascan origin that means "Place of the snakes".

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