Originally formed in 1969, the 65th Aggressor Squadron began flying the F-5E Tiger II in 1975. They provided aggressor training to US Air Force fighter crews participating in Red Flag and other exercises at the extensive Nellis Air Force Range. The 65th AGRS flew its last aggressor mission on April 7, 1989, before being deactivated. Re-activated on September 15, 2005, as a component of the 57th Adversary Tactics Group, the 65th AGRS is assigned 24 F-15 aircraft, painted in camouflage schemes identical to those observed on Russian-manufactured Su-27 Flanker fighters and operates in conjunction with the 64th Aggressor Squadron, which performs a similar task using F-16s.
On July 30, 2008, one pilot was killed and another injured when their F-15 crashed into the ground during a training mission.